How Do You Spell IN SITU NICK END LABELING?

Pronunciation: [ɪn sˈɪtuː nˈɪk ˈɛnd lˈe͡ɪbə͡lɪŋ] (IPA)

The term "In Situ Nick End Labeling" refers to a lab technique that labels the ends of broken DNA strands with fluorescent molecules. The spelling of this term can be unpacked using phonetic transcription, which breaks down each sound in the word into its corresponding phoneme. In IPA, "In Situ Nick End Labeling" is spelled: [ɪnˈsɪtu nɪk ɛnd ˈleɪblɪŋ]. This technique is commonly used to identify areas of DNA damage or cell death in biological samples.

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IN SITU NICK END LABELING Meaning and Definition

  1. In Situ Nick End Labeling (ISEL) is a technique used in molecular biology and pathology to identify cellular DNA fragmentation. It is commonly employed to detect and visualize DNA breaks that occur during processes such as apoptosis, necrosis, or DNA damage in cells or tissues.

    The term "in situ" refers to the fact that this labeling method is performed directly within the intact tissue or cell sample, allowing for the detection of DNA fragmentation in its native state. The labeling process involves the addition of a labeled nucleotide, which can be biotinylated or fluorescently labeled, to the free 3'-OH DNA ends generated by DNA breaks.

    Nick end labeling specifically refers to the addition of the labeled nucleotide to the nicked or broken DNA ends. The labeled nucleotide is incorporated by the enzyme terminal deoxynucleotidyl transferase (TdT), which catalyzes the addition of nucleotides to the 3'-OH DNA ends in a template-independent manner.

    Upon completion of the labeling reaction, the labeled DNA fragments can be easily visualized and detected using techniques such as fluorescence microscopy or antigen detection methods. The intensity and pattern of labeling provide valuable information regarding the extent and distribution of DNA fragmentation within the studied sample.

    In summary, In Situ Nick End Labeling (ISEL) is a technique used to identify and visualize DNA fragmentation in intact cells or tissues, helping to gain insights into various biological processes and pathological conditions characterized by DNA damage or cell death.

Common Misspellings for IN SITU NICK END LABELING

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